Modified ELISA Systems for Biomedical Analysis
These combined projects aimed to develop new generation modified ELISA systems to enhance performance compared to the traditional ELISA method. Using of aptamers as bio-recognition elements and the modification of critical steps with nanomaterials are the two main options that were evaluated in these projects.
Development of Novel Analyzing Tools for Quantitative Detection of SARS-CoV-2 Antibodies
In this group of projects, quantitative, sensitive, and selective analysis systems will be developed for IgG and IgM-type immunoglobulins produced against SARS-CoV-2. An electrochemical lateral flow test system and a portable optical detection system will be developed. The analysis systems will be an easy and fast end product that can be put into service in every environment that is needed.
Aptamer Selection and Electrochemical Detection System
This project aimed to develop an aptamer-based direct electrochemical oxidation analysis system for rapid and real-time detection of parathyroid hormone (PTH), which is important in showing the success of surgical procedures for minimally invasive parathyroidectomy. The specific aptamer for the PTH target, obtained by the in vitro selection process called SELEX, will be the first in the literature. The electrochemical test system, which has easy-to-use and portability features and does not require high electrical power, will make this biosensor device to be used at the bedside in the operating room or in any other area where it is needed.
Smart Phone Assisted Paper-based Analyzing Tools
In this group of research, we present the development of a paper-based, low-cost, sensitive, quantitative, and stable platform proper for smartphone-assisted colorimetric detection of various targets. The paper-based devices were used to detect ATP degradation products such as hypoxanthine (Hx) and inosine during the diagnosis of muscle injuries related diseases. The smartphone-assisted system also was used to observe the fresh fish product to eliminate people’s concerns while consuming seafood.
Aptamer/ Molecularly Imprinted Polymer (MIP)-Based Biosensor Systems
A molecularly imprinted polymer (MIP) and DNA aptamers may detect target molecules with high sensitivity and selectivity by simulating the structure and function of antibodies and biological receptors. Combining MIPs and aptamers could be an excellent way to create hybrid alternatives with enhanced qualities and desirable characteristics. With this motivation, a new method was developed to produce Aptamer/MIP hybrids for protein biomarker detection that could be simple and repeatable.